LOCUS HPV16R 7906 bp ds-DNA circular VRL 15-APR-1991 DEFINITION Human papillomavirus type 16 (HPV16), complete genome. ACCESSION KEYWORDS circular; complete genome. SOURCE Papilloma virus type 16 DNA recovered from a human invasive cervical carcinoma. ORGANISM Human papillomavirus type 16 Viridae; ds-DNA nonenveloped viruses; Papovaviridae; Papillomavirus. REFERENCE 1 (bases 1 to 7906) AUTHORS Seedorf,K., Kraemmer,G., Duerst,M., Suhai,S., and Roewekamp,W.G. TITLE Human papillomavirus type 16 DNA sequence JOURNAL Virology 145, 181-185 (1985) REFERENCE 2 (base 1139; revision) AUTHORS Matsukura,T., Kanda,T., Furuno,A., Yoshikawa,H., Kawana,T., and Yoshiike,K. TITLE Cloning of monomeric human papillomavirus type 16 DNA integrated within cell DNA from a cervical carcinoma. JOURNAL J. Virol. 58, 979-82 (1986) REFERENCE 3 (base 1139; revision) AUTHORS Baker,C.C., Phelps,W.C., Lindgren,V., Braun,M.J., Gonda,M.A., and Howley,P.M. TITLE Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. JOURNAL J. Virol. 61, 962-71 (1987) REFERENCE 4 (base 3907; revision) AUTHORS Bubb,V., McCance,D.J., and Schlegel,R. TITLE DNA sequence of the HPV-16 E5 ORF and the structural conservation of its encoded protein. JOURNAL Virology 163, 243-6 (1988) REFERENCE 5 (base 3907; revision) AUTHORS Halbert,C.L., and Galloway,D.A. TITLE Identification of the E5 open reading frame of human papillomavirus type 16. JOURNAL J. Virol. 62, 1071-5 (1988) REFERENCE 6 (base 4365; revision) AUTHORS Baker, C.C. TITLE The Genomes of the Papillomaviruses JOURNAL Genetic Maps: Locus Maps of Complex Genomes, 6th edition, Book 1: Viruses. S.J. O'Brien ed. Cold Spring Harbor Laboratory Press. REFERENCE 7 (base 4365; revision) AUTHORS Xi,S.Z., and Banks,L.M. TITLE Baculovirus expression of the human papillomavirus type 16 capsid proteins: detection of L1-L2 protein complexes. JOURNAL J. Gen. Virol. 72, 2981-8 (1991). REFERENCE 8 (bases 6242 and 6903-6905, deletion of GAT at 6952; revision) AUTHORS Kirnbauer,R., Taub,J., Greenstone,H., Roden,R., Durst,M., Gissmann,L., Lowy,D.R., and Schiller,J.T. TITLE Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles. JOURNAL J. Virol. 67, 6929-36 (1993) REFERENCE 9 (bases 6903-6905, deletion of GAT at 6952; revision) AUTHORS Parton,A. TITLE Nucleotide sequence of the HPV16 L1 open reading frame. JOURNAL Nucl. Acids Res. 18, 3631 (1990) REFERENCE 10 (base 7434; revision) AUTHORS Bernard,H.-U. TITLE Personal communication, 2-6-95. JOURNAL Unpublished REFERENCE 11 (base 7863; revision) AUTHORS Bernard,H.-U. TITLE Personal communication, 2-22-95. JOURNAL Unpublished REFERENCE 12 (base 7863; revision) AUTHORS Romanczuk,H., Thierry,F., and Howley,P.M. TITLE Mutational analysis of cis elements involved in E2 modulation of human papillomavirus type 16 P97 and type 18 P105 promoters. JOURNAL J. Virol. 64, 2849-59 (1990) COMMENT In developing countries, cancer of the cervix is responsible for 24% of all cancers in women. In these areas, it is the most frequent female malignancy. In developed countries, it ranks behind cancers of the breast, lung, uterus, and ovaries and accounts for 7% of all female cancers. HPV-16 is most often found in lesions of the genital mucosa which may have a risk for malignant progression. Estimates indicate that HPV-16 has been recovered from about 50-60% of all invasive cervical cancers. Studies show that the predominance of HPV-16 in high-grade anogenital lesions and invasive cancers is evident also for the vulva, the penis, and the anus. HPV-16 has also been found in approximately 80% of the Bowenoid papulosis lesions (premalignant). In contrast to the extrachromosomal form of the viral genome found in skin carcinomas of EV patients, the viral genome of HPV16 is frequently found integrated into the cellular genome in genital tract cancers. The integration of the genome requires linearization of the genome which most often occurs in the E1/E2 region. This results in the inactivation of the E2 repressor of the E6 and E7 genes. With repression removed, the E6 and E7 genes are able to immortalize keratinocytes. The DNA of HPV-16 has been molecularly cloned from a genomic library of an invasive cervical carcinoma in bateriophage lambda. The sense strand is shown, with a numbering system matching the first 60 bp of HPV1a, HPV6b and BPV1. The designation of the early and late reading frames of HPV-16 is based solely upon homology comparison with HPV-6b and BPV-1. Two TATA boxes have been identified upstream from the E6 open reading frame, at bp 17 and 65, and another upstream from the L1 coding region, at bp 4291. A potential CAT box precedes the TATA boxes near the E6 region, at bp 7898. Polyadenylation signals can be found at the end of the early and late gene regions, at bp 4215 and 7262. Open reading frame E4 does not have a translation initiation codon, which is present in most other sequenced papilloma viruses. The originally published sequence of [1] contained several sequencing errors which have since been pointed out by other authors ([4]-[12]). These changes, which have been incorporated into the present entry, are as follows: insertion of 't' at bp 3907; substitution of 't' for 'a' at bp 4365; insertion of 'cat' at bp 6903; deletion of 'gat' at bp 6952; substitution of 'cgg' for 'gc' at bp 7434; deletion of 'a' at bp 7863 (nucleotide positions given in terms of the corrected sequence presented herein). In addition to these sequencing errors, the following differences between the originally sequenced clone and the wild-type HPV-16 DNA have been noted ([2] and [3]) and are incorporated into the present sequence: insertion of 'g' in wild-type at bp 1139; substitution of 'g' for 'c' in wild-type at bp 6242. FEATURES Location/Qualifiers TATA_signal 17..23 TATA_signal 65..71 CDS 83..559 /note="E6 ORF from 65 to 559" /product="transforming protein" /gene="E6" /note="putative" /codon_start=1 /translation="MHQKRTAMFQDPQERPRKLPQLCTELQTTIHDIILECVYCKQQL LRREVYDFAFRDLCIVYRDGNPYAVCDKCLKFYSKISEYRHYCYSLYGTTLEQQYNKP LCDLLIRCINCQKPLCPEEKQRHLDKKQRFHNIRGRWTGRCMSCCRSSRTRRETQL" CDS 562..858 /note="E7 ORF from 544 to 858" /product="transforming protein" /gene="E7" /note="putative" /codon_start=1 /translation="MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQ AEPDRAHYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP" CDS 865..2814 /note="E1 ORF from 859 to 2814" /product="replication protein" /gene="E1" /note="putative" /codon_start=1 /translation="MADPAGTNGEEGTGCNGWFYVEAVVEKKTGDAISDDENENDSDT GEDLVDFIVNDNDYLTQAETETAHALFTAQEAKQHRDAVQVLKRKYLGSPLSDISGCV DNNISPRLKAICIEKQSRAAKRRLFESEDSGYGNTEVETQQMLQVEGRHETETPCSQY SGGSGGGCSQYSSGSGGEGVSERHTICQTPLTNILNVLKTSNAKAAMLAKFKELYGVS FSELVRPFKSNKSTCCDWCIAAFGLTPSIADSIKTLLQQYCLYLHIQSLACSWGMVVL LLVRYKCGKNRETIEKLLSKLLCVSPMCMMIEPPKLRSTAAALYWYKTGISNISEVYG DTPEWIQRQTVLQHSFNDCTFELSQMVQWAYDNDIVDDSEIAYKYAQLADTNSNASAF LKSNSQAKIVKDCATMCRHYKRAEKKQMSMSQWIKYRCDRVDDGGDWKQIVMFLRYQG VEFMSFLTALKRFLQGIPKKNCILLYGAANTGKSLFGMSLMKFLQGSVICFVNSKSHF WLQPLADAKIGMLDDATVPCWNYIDDNLRNALDGNLVSMDVKHRPLVQLKCPPLLITS NINAGTDSRWPYLHNRLVVFTFPNEFPFDENGNPVYELNDKNWKSFFSRTWSRLSLHE DEDKENDGDSLPTFKCVSGQNTNTL" misc_difference 1139 /note="inserted G in wild-type absent from clone of [1]; makes full E1 ORF" /citation=[2] /citation=[3] CDS 2756..3853 /note="E2 ORF from 2726 to 3853" /product="regulatory protein" /gene="E2" /note="putative" /codon_start=1 /translation="METLCQRLNVCQDKILTHYENDSTDLRDHIDYWKHMRLECAIYY KAREMGFKHINHQVVPTLAVSKNKALQAIELQLTLETIYNSQYSNEKWTLQDVSLEVY LTAPTGCIKKHGYTVEVQFDGDICNTMHYTNWTHIYICEEASVTVVEGQVDYYGLYYV HEGIRTYFVQFKDDAEKYSKNKVWEVHAGGQVILCPTSVFSSNEVSSPEIIRQHLANH PAATHTKAVALGTEETQTTIQRPRSEPDTGNPCHTTKLLHRDSVDSAPILTAFNSSHK GRINCNSNTTPIVHLKGDANTLKCLRYRFKKHCTLYTAVSSTWHWTGHNVKHKSAIVT LTYDSEWQRDQFLSQVKIPKTITVSTGFMSI" CDS <3333..3620 /note="E4 ORF from 3333 to 3620" /gene="E4" /note="putative" /codon_start=1 /translation="YYVLHLCLAATKYPLLKLLGSTWPTTPPRPIPKPSPWAPKKHRR LSSDQDQSQTPETPATPLSCCTETQWTVLQSSLHLTAHTKDGLTVIVTLHP" CDS 3850..4101 /note="E5 ORF from 3808 to 4101" /gene="E5" /note="putative" /codon_start=1 /translation="MTNLDTASTTLLACFLLCFCVLLCVCLLIRPLLLSVSTYTSLII LVLLLWITAASAFRCFIVYIIFVYIPLFLIHTHARFLIT" misc_difference 3907 /note="inserted T absent from original sequence" /citation=[4] /citation=[5] polyA_signal 4215..4220 /note="putative" CDS 4237..5658 /note="L2 ORF from 4135 to 5658" /product="minor capsid protein" /gene="L2" /note="putative" /codon_start=1 /translation="MRHKRSAKRTKRASATQLYKTCKQAGTCPPDIIPKVEGKTIADQ ILQYGSMGVFFGGLGIGTGSGTGGRTGYIPLGTRPPTATDTLAPVRPPLTVDPVGPSD PSIVSLVEETSFIDAGAPTSVPSIPPDVSGFSITTSTDTTPAILDINNTVTTVTTHNN PTFTDPSVLQPPTPAETGGHFTLSSSTISTHNYEEIPMDTFIVSTNPNTVTSSTPIPG SRPVARLGLYSRTTQQVKVVDPAFVTTPTKLITYDNPAYEGIDVDNTLYFSSNDNSIN IAPDPDFLDIVALHRPALTSRRTGIRYSRIGNKQTLRTRSGKSIGAKVHYYYDLSTID PAEEIELQTITPSTYTTTSHAASPTSINNGLYDIYADDFITDTSTTPVPSVPSTSLSG YIPANTTIPFGGAYNIPLVSGPDIPINITDQAPSLIPIVPGSPQYTIIADAGDFYLHP SYYMLRKRRKRLPYFFSDVSLAA" TATA_signal 4291..4297 misc_difference 4365 /note="A of original sequence changed to T" /citation=[6] /citation=[7] CDS 5561..7156 /note="L1 ORF from 5528 to 7156" /product="major capsid protein" /gene="L1" /note="putative" /codon_start=1 /translation="MQVTFIYILVITCYENDVNVYHIFFQMSLWLPSEATVYLPPVPV SKVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIH LPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASA YAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTNVAVNPGDCPPLELIN TVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYL RREQMFVRHLFNRAGTVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFN KPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRH GEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQ AIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQAGLKAKPKFTL GKRKATPTTSSTSTTAKRKKRKL" misc_difference 6242 /note="wild-type shows G where original clone had C" /citation=[8] misc_difference 6903..6905 /note="inserted CAT absent from original sequence" /citation=[8] /citation=[9] misc_difference 6952 /note="GAT in original sequence deleted" /citation=[8] /citation=[9] polyA_signal 7262..7267 misc_difference 7434 /note="GC in original sequence changed to CGG" /citation=[10] misc_difference 7861 /note="A in original sequence deleted" /citation=[11] /citation=[12] source 1..7906 /organism="Human papillomavirus type 16" BASE COUNT 2599 a 1377 c 1511 g 2419 t ORIGIN 83 bp upstream from beginning of E6 cds. 1 actacaataa ttcatgtata aaactaaggg cgtaaccgaa atcggttgaa ccgaaaccgg 61 ttagtataaa agcagacatt ttatgcacca aaagagaact gcaatgtttc aggacccaca 121 ggagcgaccc agaaagttac cacagttatg cacagagctg caaacaacta tacatgatat 181 aatattagaa tgtgtgtact gcaagcaaca gttactgcga cgtgaggtat atgactttgc 241 ttttcgggat ttatgcatag tatatagaga tgggaatcca tatgctgtat gtgataaatg 301 tttaaagttt tattctaaaa ttagtgagta tagacattat tgttatagtt tgtatggaac 361 aacattagaa cagcaataca acaaaccgtt gtgtgatttg ttaattaggt gtattaactg 421 tcaaaagcca ctgtgtcctg aagaaaagca aagacatctg gacaaaaagc aaagattcca 481 taatataagg ggtcggtgga ccggtcgatg tatgtcttgt tgcagatcat caagaacacg 541 tagagaaacc cagctgtaat catgcatgga gatacaccta cattgcatga atatatgtta 601 gatttgcaac cagagacaac tgatctctac tgttatgagc aattaaatga cagctcagag 661 gaggaggatg aaatagatgg tccagctgga caagcagaac cggacagagc ccattacaat 721 attgtaacct tttgttgcaa gtgtgactct acgcttcggt tgtgcgtaca aagcacacac 781 gtagacattc gtactttgga agacctgtta atgggcacac taggaattgt gtgccccatc 841 tgttctcaga aaccataatc taccatggct gatcctgcag gtaccaatgg ggaagagggt 901 acgggatgta atggatggtt ttatgtagag gctgtagtgg aaaaaaaaac aggggatgct 961 atatcagatg acgagaacga aaatgacagt gatacaggtg aagatttggt agattttata 1021 gtaaatgata atgattattt aacacaggca gaaacagaga cagcacatgc gttgtttact 1081 gcacaggaag caaaacaaca tagagatgca gtacaggttc taaaacgaaa gtatttgggt 1141 agtccactta gtgatattag tggatgtgta gacaataata ttagtcctag attaaaagct 1201 atatgtatag aaaaacaaag tagagctgca aaaaggagat tatttgaaag cgaagacagc 1261 gggtatggca atactgaagt ggaaactcag cagatgttac aggtagaagg gcgccatgag 1321 actgaaacac catgtagtca gtatagtggt ggaagtgggg gtggttgcag tcagtacagt 1381 agtggaagtg ggggagaggg tgttagtgaa agacacacta tatgccaaac accacttaca 1441 aatattttaa atgtactaaa aactagtaat gcaaaggcag caatgttagc aaaatttaaa 1501 gagttatacg gggtgagttt ttcagaatta gtaagaccat ttaaaagtaa taaatcaacg 1561 tgttgcgatt ggtgtattgc tgcatttgga cttacaccca gtatagctga cagtataaaa 1621 acactattac aacaatattg tttatattta cacattcaaa gtttagcatg ttcatgggga 1681 atggttgtgt tactattagt aagatataaa tgtggaaaaa atagagaaac aattgaaaaa 1741 ttgctgtcta aactattatg tgtgtctcca atgtgtatga tgatagagcc tccaaaattg 1801 cgtagtacag cagcagcatt atattggtat aaaacaggta tatcaaatat tagtgaagtg 1861 tatggagaca cgccagaatg gatacaaaga caaacagtat tacaacatag ttttaatgat 1921 tgtacatttg aattatcaca gatggtacaa tgggcctacg ataatgacat agtagacgat 1981 agtgaaattg catataaata tgcacaattg gcagacacta atagtaatgc aagtgccttt 2041 ctaaaaagta attcacaggc aaaaattgta aaggattgtg caacaatgtg tagacattat 2101 aaacgagcag aaaaaaaaca aatgagtatg agtcaatgga taaaatatag atgtgatagg 2161 gtagatgatg gaggtgattg gaagcaaatt gttatgtttt taaggtatca aggtgtagag 2221 tttatgtcat ttttaactgc attaaaaaga tttttgcaag gcatacctaa aaaaaattgc 2281 atattactat atggtgcagc taacacaggt aaatcattat ttggtatgag tttaatgaaa 2341 tttctgcaag ggtctgtaat atgttttgta aattctaaaa gccatttttg gttacaacca 2401 ttagcagatg ccaaaatagg tatgttagat gatgctacag tgccctgttg gaactacata 2461 gatgacaatt taagaaatgc attggatgga aatttagttt ctatggatgt aaagcataga 2521 ccattggtac aactaaaatg ccctccatta ttaattacat ctaacattaa tgctggtaca 2581 gattctaggt ggccttattt acataataga ttggtggtgt ttacatttcc taatgagttt 2641 ccatttgacg aaaacggaaa tccagtgtat gagcttaatg ataagaactg gaaatccttt 2701 ttctcaagga cgtggtccag attaagtttg cacgaggacg aggacaagga aaacgatgga 2761 gactctttgc caacgtttaa atgtgtgtca ggacaaaata ctaacacatt atgaaaatga 2821 tagtacagac ctacgtgacc atatagacta ttggaaacac atgcgcctag aatgtgctat 2881 ttattacaag gccagagaaa tgggatttaa acatattaac caccaagtgg tgccaacact 2941 ggctgtatca aagaataaag cattacaagc aattgaactg caactaacgt tagaaacaat 3001 atataactca caatatagta atgaaaagtg gacattacaa gacgttagcc ttgaagtgta 3061 tttaactgca ccaacaggat gtataaaaaa acatggatat acagtggaag tgcagtttga 3121 tggagacata tgcaatacaa tgcattatac aaactggaca catatatata tttgtgaaga 3181 agcatcagta actgtggtag agggtcaagt tgactattat ggtttatatt atgttcatga 3241 aggaatacga acatattttg tgcagtttaa agatgatgca gaaaaatata gtaaaaataa 3301 agtatgggaa gttcatgcgg gtggtcaggt aatattatgt cctacatctg tgtttagcag 3361 caacgaagta tcctctcctg aaattattag gcagcacttg gccaaccacc ccgccgcgac 3421 ccataccaaa gccgtcgcct tgggcaccga agaaacacag acgactatcc agcgaccaag 3481 atcagagcca gacaccggaa acccctgcca caccactaag ttgttgcaca gagactcagt 3541 ggacagtgct ccaatcctca ctgcatttaa cagctcacac aaaggacgga ttaactgtaa 3601 tagtaacact acacccatag tacatttaaa aggtgatgct aatactttaa aatgtttaag 3661 atatagattt aaaaagcatt gtacattgta tactgcagtg tcgtctacat ggcattggac 3721 aggacataat gtaaaacata aaagtgcaat tgttacactt acatatgata gtgaatggca 3781 acgtgaccaa tttttgtctc aagttaaaat accaaaaact attacagtgt ctactggatt 3841 tatgtctata tgacaaatct tgatactgca tccacaacat tactggcgtg ctttttgctt 3901 tgcttttgtg tgcttttgtg tgtctgccta ttaatacgtc cgctgctttt gtctgtgtct 3961 acatacacat cattaataat attggtatta ctattgtgga taacagcagc ctctgcgttt 4021 aggtgtttta ttgtatatat tatatttgtt tatataccat tatttttaat acatacacat 4081 gcacgctttt taattacata atgtatatgt acataatgta attgttacat ataattgttg 4141 tataccataa cttactattt tttctttttt attttcatat ataatttttt tttttgtttg 4201 tttgtttgtt ttttaataaa ctgttattac ttaacaatgc gacacaaacg ttctgcaaaa 4261 cgcacaaaac gtgcatcggc tacccaactt tataaaacat gcaaacaggc aggtacatgt 4321 ccacctgaca ttatacctaa ggttgaaggc aaaactattg ctgatcaaat attacaatat 4381 ggaagtatgg gtgtattttt tggtgggtta ggaattggaa cagggtcggg tacaggcgga 4441 cgcactgggt atattccatt gggaacaagg cctcccacag ctacagatac acttgctcct 4501 gtaagacccc ctttaacagt agatcctgtg ggcccttctg atccttctat agtttcttta 4561 gtggaagaaa ctagttttat tgatgctggt gcaccaacat ctgtaccttc cattccccca 4621 gatgtatcag gatttagtat tactacttca actgatacca cacctgctat attagatatt 4681 aataatactg ttactactgt tactacacat aataatccca ctttcactga cccatctgta 4741 ttgcagcctc caacacctgc agaaactgga gggcatttta cactttcatc atccactatt 4801 agtacacata attatgaaga aattcctatg gatacattta ttgttagcac aaaccctaac 4861 acagtaacta gtagcacacc cataccaggg tctcgcccag tggcacgcct aggattatat 4921 agtcgcacaa cacaacaggt taaagttgta gaccctgctt ttgtaaccac tcccactaaa 4981 cttattacat atgataatcc tgcatatgaa ggtatagatg tggataatac attatatttt 5041 tctagtaatg ataatagtat taatatagct ccagatcctg actttttgga tatagttgct 5101 ttacataggc cagcattaac ctctaggcgt actggcatta ggtacagtag aattggtaat 5161 aaacaaacac tacgtactcg tagtggaaaa tctataggtg ctaaggtaca ttattattat 5221 gatttaagta ctattgatcc tgcagaagaa atagaattac aaactataac accttctaca 5281 tatactacca cttcacatgc agcctcacct acttctatta ataatggatt atatgatatt 5341 tatgcagatg actttattac agatacttct acaaccccgg taccatctgt accctctaca 5401 tctttatcag gttatattcc tgcaaataca acaattcctt ttggtggtgc atacaatatt 5461 cctttagtat caggtcctga tatacccatt aatataactg accaagctcc ttcattaatt 5521 cctatagttc cagggtctcc acaatataca attattgctg atgcaggtga cttttattta 5581 catcctagtt attacatgtt acgaaaacga cgtaaacgtt taccatattt tttttcagat 5641 gtctctttgg ctgcctagtg aggccactgt ctacttgcct cctgtcccag tatctaaggt 5701 tgtaagcacg gatgaatatg ttgcacgcac aaacatatat tatcatgcag gaacatccag 5761 actacttgca gttggacatc cctattttcc tattaaaaaa cctaacaata acaaaatatt 5821 agttcctaaa gtatcaggat tacaatacag ggtatttaga atacatttac ctgaccccaa 5881 taagtttggt tttcctgaca cctcatttta taatccagat acacagcggc tggtttgggc 5941 ctgtgtaggt gttgaggtag gtcgtggtca gccattaggt gtgggcatta gtggccatcc 6001 tttattaaat aaattggatg acacagaaaa tgctagtgct tatgcagcaa atgcaggtgt 6061 ggataataga gaatgtatat ctatggatta caaacaaaca caattgtgtt taattggttg 6121 caaaccacct ataggggaac actggggcaa aggatcccca tgtaccaatg ttgcagtaaa 6181 tccaggtgat tgtccaccat tagagttaat aaacacagtt attcaggatg gtgatatggt 6241 tgatactggc tttggtgcta tggactttac tacattacag gctaacaaaa gtgaagttcc 6301 actggatatt tgtacatcta tttgcaaata tccagattat attaaaatgg tgtcagaacc 6361 atatggcgac agcttatttt tttatttacg aagggaacaa atgtttgtta gacatttatt 6421 taatagggct ggtactgttg gtgaaaatgt accagacgat ttatacatta aaggctctgg 6481 gtctactgca aatttagcca gttcaaatta ttttcctaca cctagtggtt ctatggttac 6541 ctctgatgcc caaatattca ataaacctta ttggttacaa cgagcacagg gccacaataa 6601 tggcatttgt tggggtaacc aactatttgt tactgttgtt gatactacac gcagtacaaa 6661 tatgtcatta tgtgctgcca tatctacttc agaaactaca tataaaaata ctaactttaa 6721 ggagtaccta cgacatgggg aggaatatga tttacagttt atttttcaac tgtgcaaaat 6781 aaccttaact gcagacgtta tgacatacat acattctatg aattccacta ttttggagga 6841 ctggaatttt ggtctacaac ctcccccagg aggcacacta gaagatactt ataggtttgt 6901 aacatcccag gcaattgctt gtcaaaaaca tacacctcca gcacctaaag aagatcccct 6961 taaaaaatac actttttggg aagtaaattt aaaggaaaag ttttctgcag acctagatca 7021 gtttccttta ggacgcaaat ttttactaca agcaggattg aaggccaaac caaaatttac 7081 attaggaaaa cgaaaagcta cacccaccac ctcatctacc tctacaactg ctaaacgcaa 7141 aaaacgtaag ctgtaagtat tgtatgtatg ttgaattagt gttgtttgtt gtgtatatgt 7201 ttgtatgtgc ttgtatgtgc ttgtaaatat taagttgtat gtgtgtttgt atgtatggta 7261 taataaacac gtgtgtatgt gtttttaaat gcttgtgtaa ctattgtgtc atgcaacata 7321 aataaactta ttgtttcaac acctactaat tgtgttgtgg ttattcattg tatataaact 7381 atatttgcta catcctgttt ttgttttata tatactatat tttgtagcgc cagcggccat 7441 tttgtagctt caaccgaatt cggttgcatg ctttttggca caaaatgtgt ttttttaaat 7501 agttctatgt cagcaactat ggtttaaact tgtacgtttc ctgcttgcca tgcgtgccaa 7561 atccctgttt tcctgacctg cactgcttgc caaccattcc attgtttttt acactgcact 7621 atgtgcaact actgaatcac tatgtacatt gtgtcatata aaataaatca ctatgcgcca 7681 acgccttaca taccgctgtt aggcacatat ttttggcttg ttttaactaa cctaattgca 7741 tatttggcat aaggtttaaa cttctaaggc caactaaatg tcaccctagt tcatacatga 7801 actgtgtaaa ggttagtcat acattgttca tttgtaaaac tgcacatggg tgtgtgcaaa 7861 ccgttttggg ttacacattt acaagcaact tatataataa tactaa //