ID   HPV44E6    STANDARD;  ds-DNA; VRL; 590 bp.
XX
DE   Human papillomavirus type 44 (HPV-44), E6 region.
XX
AC   M27023
XX
DT   15-SEP-1989
XX
OS   Human papillomavirus type 44 DNA recovered from a vulvar
OS   condyloma.
OC   Human papillomavirus type 44
OC   Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
OC   Papillomavirus. 
XX
RN   [1]
RP   1-590
RA   Lorincz,A.T., Quinn,A.P., Goldsborough,M.D., Schmidt,B.J. and
RA   Temple,G.F.;
RT   "Cloning and partial DNA sequencing of two new human papillomavirus
RT   types associated with condylomas and low-grade cervical neoplasia";
RL   J. Virol. 63, 2829-2834 (1989)
XX
XX   HPV-44 is a mucosatropic HPV which to date has not been detected in
XX   cervical cancer.  Prevalence studies indicate that HPV-44 and HPV-43
XX   have been found in 4% of cervical intraepithelial neoplasms, but in
XX   none of the 56 cervical cancers tested.   
XX   
XX   During the analysis of approximately 1000 anogenital tissue samples,
XX   two new HPV types, HPV-43 and HPV-44, were identified.  The complete
XX   genome of HPV-44 was recovered from a vulvar condyloma and cloned
XX   into bacteriophage lambda.  The biopsy was taken from a woman from
XX   the Detroit Michigan area.  The DNA recovered was a single 7.8 kb
XX   BamHI fragment.  Only the E6 region of the cloned sample has been
XX   sequenced, although the positions of the ORFs for the entire genome
XX   have been deduced and are consistent with the organization of DNA
XX   from HPV-6b.  A possible feature of HPV types associated with
XX   malignant lesions is the potential to produce a different E6 protein
XX   by alternative splicing.  This potential has been found in types
XX   HPV-16, HPV-18, and HPV-31.  HPV-44 has a potential E6 splice donor
XX   at nt 229, but does not contain a potential splice acceptor.   
XX   
XX
FT   KEY              Location/Qualifiers
FT    CDS             103..555
FT                    /note="putative"
FT                    /note="ORF E6 from bp 70 to 555"
FT                    /product="transforming protein"
FT                    /gene="E6"
FT                    /note="putative"
FT                    /codon_start=1
XX
SQ   SEQUENCE  590 bp;  197  a;  117  c;  124  g;  152  t;
     aataataatc taacctttac aaaaaagagg aggaaccgaa ttcggttcca accgaaaacg          60
     gttatataaa aaccagccca aaaattaagc aagcggggca taatggaaag tgcaaatgcc         120
     tccacgtctg cacaaagtat agaccagttg tgcaaggagt gcaacattcc tatgcacaat         180
     ctgcaaattt tatgcgtgtt ttgcagaaaa acgttaagta ctgcagaggt ttattcattc         240
     gcatataaac agttatatgt agtgtaccga ggaaactttc catttgcagc ctgtgccatt         300
     tgtttagaac tacaaggtaa ggtcaatcaa tttaggcatt ttaactacgc gggatatgca         360
     gtaacagtgg aagaagaaac aaataagtca attctggacg tgctgatacg ctgctatttg         420
     tgccacaaac cattgtgcca cgtggaaaag gtgcgccaca tattggacaa ggcgcgattc         480
     attaaattac aagatacctg gaagggtcgc tgcttccatt gttggacatc atgcatggaa         540
     actatactac cttaaaggaa attgttttac agctggaacc tcctgaccct                    590
//