ID   HPV43E6    STANDARD;  ds-DNA; VRL; 591 bp.
XX
DE   Human papillomavirus type 43 (HPV-43), E6 region.
XX
AC   M27022
XX
DT   15-SEP-1989
XX
OS   Human papillomavirus type 43 DNA recovered from a vulvar
OS   biopsy with hyperplasia.
OC   Human papillomavirus type 43
OC   Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
OC   Papillomavirus.
XX
RN   [1]
RP   1-591
RA   Lorincz,A.T., Quinn,A.P., Goldsborough,M.D., Schmidt,B.J. and
RA   Temple,G.F.;
RT   "Cloning and partial DNA sequencing of two new human papillomavirus
RT   types associated with condylomas and low-grade cervical neoplasia";
RL   J. Virol. 63, 2829-2834 (1989).
XX
XX   HPV-43 is a mucosatropic HPV which to date has not been detected in
XX   cervical cancer.  Prevalence studies indicate that HPV-44 and HPV-43
XX   have been found in 4% of cervical intraepithelial neoplasms, but in
XX   none of the 56 cervical cancers tested.
XX   
XX   During an analysis of approximately 1000 anogenital tissue samples,
XX   two new HPV types, HPV-43 and HPV-44, were identified.  The complete
XX   genome of HPV-43 was recovered from a vulvar biopsy and cloned into
XX   bacteriophage lambda.  The biopsy was taken from a woman living in
XX   the Detroit Michigan area.  The DNA consisted of two fragments: a 
XX   6.3 kb BamHI fragment and a 2.9 kb HindIII fragment.  The total 
XX   quantity of unique DNA was 7.6 kb.  Only the E6 region of the cloned
XX   sample has been sequenced, although all positions of the ORFs
XX   have been deduced and are consistent with the organization of DNA
XX   from HPV-6b.  A possible feature of HPV types associated with
XX   malignant lesions is the potential to produce a different E6
XX   protein by alternative splicing.  This potential has been found in
XX   types HPV-16, HPV-18, and HPV-31.  HPV-43 has both the potential E6
XX   splice donor site at nt 233 and the potential splice acceptor at
XX   nt 413.
XX   
XX
FT   KEY              Location/Qualifiers
FT    CDS             107..574
FT                    /note="putative"
FT                    /note="ORF E6 from bp 32 to 574"
FT                    /product="transforming protein"
FT                    /gene="E6"
FT                    /note="putative"
FT                    /codon_start=1
XX
SQ   SEQUENCE  591 bp;  189  a;  110  c;  133  g;  159  t;
     attactaaca attattatac ttgtagttta agggtgggac cgaaaacggt ccgaccgaaa          60
     gcggtacata tataaaccac ccaaaaacca tagcttgtgg ggcataatgt ctgcacgtag         120
     ctgctcccaa aacgcacgga ctatatttga gttgtgtgat gagtgtaaca taactttgcc         180
     tactctgcaa attgggtgca tattttgcaa gaagtggtta cttaccacgg aagtattatc         240
     gtttgcattt agagatttaa gggttgtgtg gcgcgacgga tatccgtttg ctgcatgctt         300
     ggcctgtcta cagtttcatg gaaaaataag tcaatatagg cactttgact acgcagcata         360
     tgcagatact gtagaagaag aaacaaagca aacagtgttt gatttgtgca ttagatgctg         420
     taagtgccac aagccattat caccagtgga aaaagtacag catattgtgc aaaaggcaca         480
     attctttaaa atacatagcg tgtggaaagg atactgctta cattgctgga aatcatgcat         540
     ggaaaaacgc cgacgatcag agactatgtg ctaactatgc aaccagaacc t                  591
//