ID   HPV16R	STANDARD;	ds-DNA;	VRL;	7906 bp.
XX
DE   Human papillomavirus type 16 (HPV16), complete genome. 
XX
AC   <not yet entered in GenBank>
XX
DT   15-APR-1991
XX
OS   Papilloma virus type 16 DNA recovered from a human invasive cervical 
OS   carcinoma. 
OC   Human papillomavirus type 16;Viridae; ds-DNA nonenveloped viruses; 
OC   Papovaviridae;Papillomavirus.
XX
RN   [1]
RP   1 - 7906
RA   Seedorf,K., Kraemmer,G., Duerst,M., Suhai,S., and  Roewekamp,W.G;
RT   "Human papillomavirus type 16 DNA sequence;"
RL   Virology 145, 181-185 (1985).
XX
RN   [2]
RP   base 1139; revision
RA   Matsukura,T., Kanda,T., Furuno,A., Yoshikawa,H., Kawana,T.,and 
RA   Yoshiike,K;
RT   "Cloning of monomeric human papillomavirus type 16 DNA integrated 
RT   within cell DNA from a cervical carcinoma;"
RL   J. Virol. 58, 979-82 (1986).
XX
RN   [3]
RP   base 1139; revision
RA   Baker,C.C., Phelps,W.C., Lindgren,V., Braun,M.J., Gonda,M.A.,and 
RA   Howley,P.M;
RT   "Structural and transcriptional analysis of human papillomavirus type 
RT   16 sequences in cervical carcinoma cell lines;"
RL   J. Virol. 61, 962-71 (1987).
XX
RN   [4]
RP   base 3907; revision
RA   Bubb,V., McCance,D.J., and Schlegel,R;
RT   "DNA sequence of the HPV-16 E5 ORF and the structural conservation of 
RT   its encoded protein;"
RL   Virology 163, 243-6 (1988).
XX
RN   [5]
RP   base 3907; revision
RA   Halbert,C.L., and Galloway,D.A;
RT   "Identification of the E5 open  reading frame of human papillomavirus 
RT   type 16;"
RL   J. Virol. 62, 1071-5 (1988).
XX
RN   [6]
RP   base 4365; revision
RA   Baker, C.C;
RT   "The Genomes of the Papillomaviruses;"
RL   Genetic Maps: Locus Maps ofComplex Genomes, 6th edition, Book 1: 
RL   Viruses. S.J. O'Brien ed. Cold Spring Harbor Laboratory Press.
XX
RN   [7]
RP   base 4365; revision
RA   Xi,S.Z., and Banks,L.M;
RT   "Baculovirus expression of the human papillomavirus  type 16 capsid 
RT   proteins: detection of L1-L2 protein complexes;"
RL   J. Gen. Virol. 72, 2981-8 (1991).
XX
RN   [8]
RP   6242 - 7906
RA   Kirnbauer,R., Taub,J., Greenstone,H., Roden,R., Durst,M.,Gissmann,L., 
RA   Lowy,D.R., and Schiller,J.T;
RT   "Efficient self-assembly of human papillomavirus type 16  L1 and L1-L2 
RT   into virus-like particles;"
RL   J. Virol. 67, 6929-36 (1993).
XX
RN   [9]
RP   6903 - 7906
RA   Parton,A;
RT   "Nucleotide sequence of the HPV16 L1 open reading frame;"
RL   Nucl. Acids Res. 18, 3631 (1990).
XX
RN   [10]
RP   (base 7434; revision
RA   Bernard,H.-U;
RT   "Personal communication, 2-6-95;"
RL   Unpublished.
XX
RN   [11]
RP   (base 7863; revision
RA   Bernard,H.-U;
RT   "Personal communication, 2-22-95;"
RL   Unpublished.
XX
RN   [12]
RP   (base 7863; revision
RA   Romanczuk,H., Thierry,F., and Howley,P.M;
RT   "Mutational analysis of cis elements involved in E2 modulation of human 
RT   papillomavirus type 16 P97 and type 18 P105 promoters;"
RL   J. Virol. 64, 2849-59 (1990).
XX
XX   Created by HIV database on 31-MAY-1995 from GenBank: <not.
XX
XX   In developing countries, cancer of the cervix is responsible for
XX   24% of all cancers in women.  In these areas, it is the most
XX   frequent female malignancy.  In developed countries, it ranks
XX   behind cancers of the breast, lung, uterus, and ovaries and
XX   accounts for 7% of all female cancers.  HPV-16 is most often found
XX   in lesions of the genital mucosa which may have a risk for
XX   malignant progression.  Estimates indicate that HPV-16 has been
XX   recovered from about 50-60% of all invasive cervical cancers.
XX   Studies show that the predominance of HPV-16 in high-grade
XX   anogenital lesions and invasive cancers is evident also for the
XX   vulva, the penis, and the anus.  HPV-16 has also been found in
XX   approximately 80% of the Bowenoid papulosis lesions (premalignant).
XX   
XX   In contrast to the extrachromosomal form of the viral genome found
XX   in skin carcinomas of EV patients, the viral genome of HPV16 is
XX   frequently found integrated into the cellular genome in genital
XX   tract cancers.  The integration of the genome requires
XX   linearization of the genome which most often occurs in the E1/E2
XX   region.  This results in the inactivation of the E2 repressor of
XX   the E6 and E7 genes.  With repression removed, the E6 and E7 genes
XX   are able to immortalize keratinocytes.
XX   
XX   The DNA of HPV-16 has been molecularly cloned from a genomic
XX   library of an invasive cervical carcinoma in bateriophage lambda.
XX   The sense strand is shown, with a numbering system matching the 
XX   first 60 bp of HPV1a, HPV6b and BPV1.  The designation of the early
XX   and late reading frames of HPV-16 is based solely upon homology
XX   comparison with HPV-6b and BPV-1.  Two TATA boxes have been
XX   identified upstream from the E6 open reading frame, at bp 17 and 65,
XX   and another upstream from the L1 coding region, at bp 4291.  A 
XX   potential CAT box precedes the TATA boxes near the E6 region, at bp 
XX   7898.  Polyadenylation signals can be found at the end of the early 
XX   and late gene regions, at bp 4215 and 7262.  Open reading
XX   frame E4 does not have a translation initiation codon, which
XX   is present in most other sequenced papilloma viruses.
XX
XX   The originally published sequence of [1] contained several sequencing
XX   errors which have since been pointed out by other authors ([4]-[12]).
XX   These changes, which have been incorporated into the present entry,
XX   are as follows: insertion of 't' at bp 3907; substitution of 't' for
XX   'a' at bp 4365; insertion of 'cat' at bp 6903; deletion of 'gat' at
XX   bp 6952; substitution of 'cgg' for 'gc' at bp 7434; deletion of 'a'
XX   at bp 7863 (nucleotide positions given in terms of the corrected
XX   sequence presented herein).  In addition to these sequencing errors,
XX   the following differences between the originally sequenced clone and
XX   the wild-type HPV-16 DNA have been noted ([2] and [3]) and are
XX   incorporated into the present sequence: insertion of 'g' in wild-type
XX   at bp 1139; substitution of 'g' for 'c' in wild-type at bp 6242.
XX
XX
FT  KEY              Location/Qualifiers
FT   TATA_signal     17..23
FT   TATA_signal     65..71
FT   CDS             83..559
FT                   /note="E6 ORF from 65 to 559"
FT                   /product="transforming protein"
FT                   /gene="E6"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="MHQKRTAMFQDPQERPRKLPQLCTELQTTIHDIILECVYCKQQL
FT                   LRREVYDFAFRDLCIVYRDGNPYAVCDKCLKFYSKISEYRHYCYSLYGTTLEQQYNKP
FT                   LCDLLIRCINCQKPLCPEEKQRHLDKKQRFHNIRGRWTGRCMSCCRSSRTRRETQL"
FT   CDS             562..858
FT                   /note="E7 ORF from 544 to 858"
FT                   /product="transforming protein"
FT                   /gene="E7"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQ
FT                   AEPDRAHYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP"
FT   CDS             865..2814
FT                   /note="E1 ORF from 859 to 2814"
FT                   /product="replication protein"
FT                   /gene="E1"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="MADPAGTNGEEGTGCNGWFYVEAVVEKKTGDAISDDENENDSDT
FT                   GEDLVDFIVNDNDYLTQAETETAHALFTAQEAKQHRDAVQVLKRKYLGSPLSDISGCV
FT                   DNNISPRLKAICIEKQSRAAKRRLFESEDSGYGNTEVETQQMLQVEGRHETETPCSQY
FT                   SGGSGGGCSQYSSGSGGEGVSERHTICQTPLTNILNVLKTSNAKAAMLAKFKELYGVS
FT                   FSELVRPFKSNKSTCCDWCIAAFGLTPSIADSIKTLLQQYCLYLHIQSLACSWGMVVL
FT                   LLVRYKCGKNRETIEKLLSKLLCVSPMCMMIEPPKLRSTAAALYWYKTGISNISEVYG
FT                   DTPEWIQRQTVLQHSFNDCTFELSQMVQWAYDNDIVDDSEIAYKYAQLADTNSNASAF
FT                   LKSNSQAKIVKDCATMCRHYKRAEKKQMSMSQWIKYRCDRVDDGGDWKQIVMFLRYQG
FT                   VEFMSFLTALKRFLQGIPKKNCILLYGAANTGKSLFGMSLMKFLQGSVICFVNSKSHF
FT                   WLQPLADAKIGMLDDATVPCWNYIDDNLRNALDGNLVSMDVKHRPLVQLKCPPLLITS
FT                   NINAGTDSRWPYLHNRLVVFTFPNEFPFDENGNPVYELNDKNWKSFFSRTWSRLSLHE
FT                   DEDKENDGDSLPTFKCVSGQNTNTL"
FT   misc_difference 1139
FT                   /note="inserted G in wild-type absent from	clone of [1];
FT                   makes full E1 ORF"
FT                   /citation=[2]
FT                   /citation=[3]
FT   CDS             2756..3853
FT                   /note="E2 ORF from 2726 to 3853"
FT                   /product="regulatory protein"
FT                   /gene="E2"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="METLCQRLNVCQDKILTHYENDSTDLRDHIDYWKHMRLECAIYY
FT                   KAREMGFKHINHQVVPTLAVSKNKALQAIELQLTLETIYNSQYSNEKWTLQDVSLEVY
FT                   LTAPTGCIKKHGYTVEVQFDGDICNTMHYTNWTHIYICEEASVTVVEGQVDYYGLYYV
FT                   HEGIRTYFVQFKDDAEKYSKNKVWEVHAGGQVILCPTSVFSSNEVSSPEIIRQHLANH
FT                   PAATHTKAVALGTEETQTTIQRPRSEPDTGNPCHTTKLLHRDSVDSAPILTAFNSSHK
FT                   GRINCNSNTTPIVHLKGDANTLKCLRYRFKKHCTLYTAVSSTWHWTGHNVKHKSAIVT
FT                   LTYDSEWQRDQFLSQVKIPKTITVSTGFMSI"
FT   CDS             <3333..3620
FT                   /note="E4 ORF from 3333 to 3620"
FT                   /gene="E4"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="YYVLHLCLAATKYPLLKLLGSTWPTTPPRPIPKPSPWAPKKHRR
FT                   LSSDQDQSQTPETPATPLSCCTETQWTVLQSSLHLTAHTKDGLTVIVTLHP"
FT   CDS             3850..4101
FT                   /note="E5 ORF from 3808 to 4101"
FT                   /gene="E5"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="MTNLDTASTTLLACFLLCFCVLLCVCLLIRPLLLSVSTYTSLII
FT                   LVLLLWITAASAFRCFIVYIIFVYIPLFLIHTHARFLIT"
FT   misc_difference 3907
FT                   /note="inserted T absent from original sequence"
FT                   /citation=[4]
FT                   /citation=[5]
FT   polyA_signal    4215..4220
FT                   /note="putative"
FT   CDS             4237..5658
FT                   /note="L2 ORF from 4135 to 5658"
FT                   /product="minor capsid protein"
FT                   /gene="L2"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="MRHKRSAKRTKRASATQLYKTCKQAGTCPPDIIPKVEGKTIADQ
FT                   ILQYGSMGVFFGGLGIGTGSGTGGRTGYIPLGTRPPTATDTLAPVRPPLTVDPVGPSD
FT                   PSIVSLVEETSFIDAGAPTSVPSIPPDVSGFSITTSTDTTPAILDINNTVTTVTTHNN
FT                   PTFTDPSVLQPPTPAETGGHFTLSSSTISTHNYEEIPMDTFIVSTNPNTVTSSTPIPG
FT                   SRPVARLGLYSRTTQQVKVVDPAFVTTPTKLITYDNPAYEGIDVDNTLYFSSNDNSIN
FT                   IAPDPDFLDIVALHRPALTSRRTGIRYSRIGNKQTLRTRSGKSIGAKVHYYYDLSTID
FT                   PAEEIELQTITPSTYTTTSHAASPTSINNGLYDIYADDFITDTSTTPVPSVPSTSLSG
FT                   YIPANTTIPFGGAYNIPLVSGPDIPINITDQAPSLIPIVPGSPQYTIIADAGDFYLHP
FT                   SYYMLRKRRKRLPYFFSDVSLAA"
FT   TATA_signal     4291..4297
FT   misc_difference 4365
FT                   /note="A of original sequence changed to T"
FT                   /citation=[6]
FT                   /citation=[7]
FT   CDS             5561..7156
FT                   /note="L1 ORF from 5528 to 7156"
FT                   /product="major capsid protein"
FT                   /gene="L1"
FT                   /note="putative"
FT                   /codon_start=1
FT                   /translation="MQVTFIYILVITCYENDVNVYHIFFQMSLWLPSEATVYLPPVPV
FT                   SKVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIH
FT                   LPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASA
FT                   YAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTNVAVNPGDCPPLELIN
FT                   TVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYL
FT                   RREQMFVRHLFNRAGTVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFN
FT                   KPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRH
FT                   GEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQ
FT                   AIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQAGLKAKPKFTL
FT                   GKRKATPTTSSTSTTAKRKKRKL"
FT   misc_difference 6242
FT                   /note="wild-type shows G where original clone had C"
FT                   /citation=[8]
FT   misc_difference 6903..6905
FT                   /note="inserted CAT absent from original sequence"
FT                   /citation=[8]
FT                   /citation=[9]
FT   misc_difference 6952
FT                   /note="GAT in original sequence deleted"
FT                   /citation=[8]
FT                   /citation=[9]
FT   polyA_signal    7262..7267
FT   misc_difference 7434
FT                   /note="GC in original sequence changed to CGG"
FT                   /citation=[10]
FT   misc_difference 7861
FT                   /note="A in original sequence deleted"
FT                   /citation=[11]
FT                   /citation=[12]
FT   source          1..7906
FT                   /organism="Human papillomavirus type 16"
XX
SQ   SEQUENCE  7906 bp;  2599 a;  1377 c;  1511 g;  2419 t;
     actacaataa ttcatgtata aaactaaggg cgtaaccgaa atcggttgaa ccgaaaccgg        60
     ttagtataaa agcagacatt ttatgcacca aaagagaact gcaatgtttc aggacccaca       120
     ggagcgaccc agaaagttac cacagttatg cacagagctg caaacaacta tacatgatat       180
     aatattagaa tgtgtgtact gcaagcaaca gttactgcga cgtgaggtat atgactttgc       240
     ttttcgggat ttatgcatag tatatagaga tgggaatcca tatgctgtat gtgataaatg       300
     tttaaagttt tattctaaaa ttagtgagta tagacattat tgttatagtt tgtatggaac       360
     aacattagaa cagcaataca acaaaccgtt gtgtgatttg ttaattaggt gtattaactg       420
     tcaaaagcca ctgtgtcctg aagaaaagca aagacatctg gacaaaaagc aaagattcca       480
     taatataagg ggtcggtgga ccggtcgatg tatgtcttgt tgcagatcat caagaacacg       540
     tagagaaacc cagctgtaat catgcatgga gatacaccta cattgcatga atatatgtta       600
     gatttgcaac cagagacaac tgatctctac tgttatgagc aattaaatga cagctcagag       660
     gaggaggatg aaatagatgg tccagctgga caagcagaac cggacagagc ccattacaat       720
     attgtaacct tttgttgcaa gtgtgactct acgcttcggt tgtgcgtaca aagcacacac       780
     gtagacattc gtactttgga agacctgtta atgggcacac taggaattgt gtgccccatc       840
     tgttctcaga aaccataatc taccatggct gatcctgcag gtaccaatgg ggaagagggt       900
     acgggatgta atggatggtt ttatgtagag gctgtagtgg aaaaaaaaac aggggatgct       960
     atatcagatg acgagaacga aaatgacagt gatacaggtg aagatttggt agattttata      1020
     gtaaatgata atgattattt aacacaggca gaaacagaga cagcacatgc gttgtttact      1080
     gcacaggaag caaaacaaca tagagatgca gtacaggttc taaaacgaaa gtatttgggt      1140
     agtccactta gtgatattag tggatgtgta gacaataata ttagtcctag attaaaagct      1200
     atatgtatag aaaaacaaag tagagctgca aaaaggagat tatttgaaag cgaagacagc      1260
     gggtatggca atactgaagt ggaaactcag cagatgttac aggtagaagg gcgccatgag      1320
     actgaaacac catgtagtca gtatagtggt ggaagtgggg gtggttgcag tcagtacagt      1380
     agtggaagtg ggggagaggg tgttagtgaa agacacacta tatgccaaac accacttaca      1440
     aatattttaa atgtactaaa aactagtaat gcaaaggcag caatgttagc aaaatttaaa      1500
     gagttatacg gggtgagttt ttcagaatta gtaagaccat ttaaaagtaa taaatcaacg      1560
     tgttgcgatt ggtgtattgc tgcatttgga cttacaccca gtatagctga cagtataaaa      1620
     acactattac aacaatattg tttatattta cacattcaaa gtttagcatg ttcatgggga      1680
     atggttgtgt tactattagt aagatataaa tgtggaaaaa atagagaaac aattgaaaaa      1740
     ttgctgtcta aactattatg tgtgtctcca atgtgtatga tgatagagcc tccaaaattg      1800
     cgtagtacag cagcagcatt atattggtat aaaacaggta tatcaaatat tagtgaagtg      1860
     tatggagaca cgccagaatg gatacaaaga caaacagtat tacaacatag ttttaatgat      1920
     tgtacatttg aattatcaca gatggtacaa tgggcctacg ataatgacat agtagacgat      1980
     agtgaaattg catataaata tgcacaattg gcagacacta atagtaatgc aagtgccttt      2040
     ctaaaaagta attcacaggc aaaaattgta aaggattgtg caacaatgtg tagacattat      2100
     aaacgagcag aaaaaaaaca aatgagtatg agtcaatgga taaaatatag atgtgatagg      2160
     gtagatgatg gaggtgattg gaagcaaatt gttatgtttt taaggtatca aggtgtagag      2220
     tttatgtcat ttttaactgc attaaaaaga tttttgcaag gcatacctaa aaaaaattgc      2280
     atattactat atggtgcagc taacacaggt aaatcattat ttggtatgag tttaatgaaa      2340
     tttctgcaag ggtctgtaat atgttttgta aattctaaaa gccatttttg gttacaacca      2400
     ttagcagatg ccaaaatagg tatgttagat gatgctacag tgccctgttg gaactacata      2460
     gatgacaatt taagaaatgc attggatgga aatttagttt ctatggatgt aaagcataga      2520
     ccattggtac aactaaaatg ccctccatta ttaattacat ctaacattaa tgctggtaca      2580
     gattctaggt ggccttattt acataataga ttggtggtgt ttacatttcc taatgagttt      2640
     ccatttgacg aaaacggaaa tccagtgtat gagcttaatg ataagaactg gaaatccttt      2700
     ttctcaagga cgtggtccag attaagtttg cacgaggacg aggacaagga aaacgatgga      2760
     gactctttgc caacgtttaa atgtgtgtca ggacaaaata ctaacacatt atgaaaatga      2820
     tagtacagac ctacgtgacc atatagacta ttggaaacac atgcgcctag aatgtgctat      2880
     ttattacaag gccagagaaa tgggatttaa acatattaac caccaagtgg tgccaacact      2940
     ggctgtatca aagaataaag cattacaagc aattgaactg caactaacgt tagaaacaat      3000
     atataactca caatatagta atgaaaagtg gacattacaa gacgttagcc ttgaagtgta      3060
     tttaactgca ccaacaggat gtataaaaaa acatggatat acagtggaag tgcagtttga      3120
     tggagacata tgcaatacaa tgcattatac aaactggaca catatatata tttgtgaaga      3180
     agcatcagta actgtggtag agggtcaagt tgactattat ggtttatatt atgttcatga      3240
     aggaatacga acatattttg tgcagtttaa agatgatgca gaaaaatata gtaaaaataa      3300
     agtatgggaa gttcatgcgg gtggtcaggt aatattatgt cctacatctg tgtttagcag      3360
     caacgaagta tcctctcctg aaattattag gcagcacttg gccaaccacc ccgccgcgac      3420
     ccataccaaa gccgtcgcct tgggcaccga agaaacacag acgactatcc agcgaccaag      3480
     atcagagcca gacaccggaa acccctgcca caccactaag ttgttgcaca gagactcagt      3540
     ggacagtgct ccaatcctca ctgcatttaa cagctcacac aaaggacgga ttaactgtaa      3600
     tagtaacact acacccatag tacatttaaa aggtgatgct aatactttaa aatgtttaag      3660
     atatagattt aaaaagcatt gtacattgta tactgcagtg tcgtctacat ggcattggac      3720
     aggacataat gtaaaacata aaagtgcaat tgttacactt acatatgata gtgaatggca      3780
     acgtgaccaa tttttgtctc aagttaaaat accaaaaact attacagtgt ctactggatt      3840
     tatgtctata tgacaaatct tgatactgca tccacaacat tactggcgtg ctttttgctt      3900
     tgcttttgtg tgcttttgtg tgtctgccta ttaatacgtc cgctgctttt gtctgtgtct      3960
     acatacacat cattaataat attggtatta ctattgtgga taacagcagc ctctgcgttt      4020
     aggtgtttta ttgtatatat tatatttgtt tatataccat tatttttaat acatacacat      4080
     gcacgctttt taattacata atgtatatgt acataatgta attgttacat ataattgttg      4140
     tataccataa cttactattt tttctttttt attttcatat ataatttttt tttttgtttg      4200
     tttgtttgtt ttttaataaa ctgttattac ttaacaatgc gacacaaacg ttctgcaaaa      4260
     cgcacaaaac gtgcatcggc tacccaactt tataaaacat gcaaacaggc aggtacatgt      4320
     ccacctgaca ttatacctaa ggttgaaggc aaaactattg ctgatcaaat attacaatat      4380
     ggaagtatgg gtgtattttt tggtgggtta ggaattggaa cagggtcggg tacaggcgga      4440
     cgcactgggt atattccatt gggaacaagg cctcccacag ctacagatac acttgctcct      4500
     gtaagacccc ctttaacagt agatcctgtg ggcccttctg atccttctat agtttcttta      4560
     gtggaagaaa ctagttttat tgatgctggt gcaccaacat ctgtaccttc cattccccca      4620
     gatgtatcag gatttagtat tactacttca actgatacca cacctgctat attagatatt      4680
     aataatactg ttactactgt tactacacat aataatccca ctttcactga cccatctgta      4740
     ttgcagcctc caacacctgc agaaactgga gggcatttta cactttcatc atccactatt      4800
     agtacacata attatgaaga aattcctatg gatacattta ttgttagcac aaaccctaac      4860
     acagtaacta gtagcacacc cataccaggg tctcgcccag tggcacgcct aggattatat      4920
     agtcgcacaa cacaacaggt taaagttgta gaccctgctt ttgtaaccac tcccactaaa      4980
     cttattacat atgataatcc tgcatatgaa ggtatagatg tggataatac attatatttt      5040
     tctagtaatg ataatagtat taatatagct ccagatcctg actttttgga tatagttgct      5100
     ttacataggc cagcattaac ctctaggcgt actggcatta ggtacagtag aattggtaat      5160
     aaacaaacac tacgtactcg tagtggaaaa tctataggtg ctaaggtaca ttattattat      5220
     gatttaagta ctattgatcc tgcagaagaa atagaattac aaactataac accttctaca      5280
     tatactacca cttcacatgc agcctcacct acttctatta ataatggatt atatgatatt      5340
     tatgcagatg actttattac agatacttct acaaccccgg taccatctgt accctctaca      5400
     tctttatcag gttatattcc tgcaaataca acaattcctt ttggtggtgc atacaatatt      5460
     cctttagtat caggtcctga tatacccatt aatataactg accaagctcc ttcattaatt      5520
     cctatagttc cagggtctcc acaatataca attattgctg atgcaggtga cttttattta      5580
     catcctagtt attacatgtt acgaaaacga cgtaaacgtt taccatattt tttttcagat      5640
     gtctctttgg ctgcctagtg aggccactgt ctacttgcct cctgtcccag tatctaaggt      5700
     tgtaagcacg gatgaatatg ttgcacgcac aaacatatat tatcatgcag gaacatccag      5760
     actacttgca gttggacatc cctattttcc tattaaaaaa cctaacaata acaaaatatt      5820
     agttcctaaa gtatcaggat tacaatacag ggtatttaga atacatttac ctgaccccaa      5880
     taagtttggt tttcctgaca cctcatttta taatccagat acacagcggc tggtttgggc      5940
     ctgtgtaggt gttgaggtag gtcgtggtca gccattaggt gtgggcatta gtggccatcc      6000
     tttattaaat aaattggatg acacagaaaa tgctagtgct tatgcagcaa atgcaggtgt      6060
     ggataataga gaatgtatat ctatggatta caaacaaaca caattgtgtt taattggttg      6120
     caaaccacct ataggggaac actggggcaa aggatcccca tgtaccaatg ttgcagtaaa      6180
     tccaggtgat tgtccaccat tagagttaat aaacacagtt attcaggatg gtgatatggt      6240
     tgatactggc tttggtgcta tggactttac tacattacag gctaacaaaa gtgaagttcc      6300
     actggatatt tgtacatcta tttgcaaata tccagattat attaaaatgg tgtcagaacc      6360
     atatggcgac agcttatttt tttatttacg aagggaacaa atgtttgtta gacatttatt      6420
     taatagggct ggtactgttg gtgaaaatgt accagacgat ttatacatta aaggctctgg      6480
     gtctactgca aatttagcca gttcaaatta ttttcctaca cctagtggtt ctatggttac      6540
     ctctgatgcc caaatattca ataaacctta ttggttacaa cgagcacagg gccacaataa      6600
     tggcatttgt tggggtaacc aactatttgt tactgttgtt gatactacac gcagtacaaa      6660
     tatgtcatta tgtgctgcca tatctacttc agaaactaca tataaaaata ctaactttaa      6720
     ggagtaccta cgacatgggg aggaatatga tttacagttt atttttcaac tgtgcaaaat      6780
     aaccttaact gcagacgtta tgacatacat acattctatg aattccacta ttttggagga      6840
     ctggaatttt ggtctacaac ctcccccagg aggcacacta gaagatactt ataggtttgt      6900
     aacatcccag gcaattgctt gtcaaaaaca tacacctcca gcacctaaag aagatcccct      6960
     taaaaaatac actttttggg aagtaaattt aaaggaaaag ttttctgcag acctagatca      7020
     gtttccttta ggacgcaaat ttttactaca agcaggattg aaggccaaac caaaatttac      7080
     attaggaaaa cgaaaagcta cacccaccac ctcatctacc tctacaactg ctaaacgcaa      7140
     aaaacgtaag ctgtaagtat tgtatgtatg ttgaattagt gttgtttgtt gtgtatatgt      7200
     ttgtatgtgc ttgtatgtgc ttgtaaatat taagttgtat gtgtgtttgt atgtatggta      7260
     taataaacac gtgtgtatgt gtttttaaat gcttgtgtaa ctattgtgtc atgcaacata      7320
     aataaactta ttgtttcaac acctactaat tgtgttgtgg ttattcattg tatataaact      7380
     atatttgcta catcctgttt ttgttttata tatactatat tttgtagcgc cagcggccat      7440
     tttgtagctt caaccgaatt cggttgcatg ctttttggca caaaatgtgt ttttttaaat      7500
     agttctatgt cagcaactat ggtttaaact tgtacgtttc ctgcttgcca tgcgtgccaa      7560
     atccctgttt tcctgacctg cactgcttgc caaccattcc attgtttttt acactgcact      7620
     atgtgcaact actgaatcac tatgtacatt gtgtcatata aaataaatca ctatgcgcca      7680
     acgccttaca taccgctgtt aggcacatat ttttggcttg ttttaactaa cctaattgca      7740
     tatttggcat aaggtttaaa cttctaaggc caactaaatg tcaccctagt tcatacatga      7800
     actgtgtaaa ggttagtcat acattgttca tttgtaaaac tgcacatggg tgtgtgcaaa      7860
     ccgttttggg ttacacattt acaagcaact tatataataa tactaa                     7906